6I2R

Crystal structure of the SucA domain of Mycobacterium smegmatis KGD (alpha-ketoglutarate decarboxylase), mutant R802A, in complex with GarA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Multifunctional 2-oxoglutarate metabolism enzyme	polymer	868	97080.5	2	UniProt (A0R2B1) Pfam (PF00676) Pfam (PF02779) Pfam (PF16870) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	2-hydroxy-3-oxoadipate synthase,HOAS,2-oxoglutarate carboxy-lyase,2-oxoglutarate decarboxylase,Alpha-ketoglutarate decarboxylase,KGD,Alpha-ketoglutarate-glyoxylate carboligase
2	B, D	Glycogen accumulation regulator GarA	polymer	115	12112.3	2	UniProt (A0QYG2) Pfam (PF00498) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
3	A, C	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
4	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A, C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	454	Chemie (HOH)

Sequence modifications

A, C: 361 - 1227 (UniProt: A0R2B1)

PDB	External Database	Details
Gly 360	-	expression tag
Ala 802	Arg 802	engineered mutation

B, D: 45 - 158 (UniProt: A0QYG2)

PDB	External Database	Details
Gly 44	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	218385.6
Non-Polymers*	Number of molecules	6
	Total formula weight	979.4
All*	Total formula weight	219365.0

*Water molecules are not included.