6I2R
Crystal structure of the SucA domain of Mycobacterium smegmatis KGD (alpha-ketoglutarate decarboxylase), mutant R802A, in complex with GarA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9334 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.745, 143.145, 167.106 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.150 - 2.200 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yic |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.150 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.089 | 0.746 |
Rpim | 0.039 | 0.526 |
Number of reflections | 113280 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 96.7 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes-Na pH 7.5, 47% v/v MPD |