6HUY

HmdII from Desulfurobacterium thermolithotrophum reconstitued with Fe-guanylylpyridinol (FeGP) cofactor and co-crystallized with methenyl-tetrahydrofolate form A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein	polymer	370	40994.0	2	UniProt (F0S2B6) Pfam (PF22616) Pfam (PF03201) In PDB	Desulfurobacterium thermolithotrophum DSM 11699
2	B, D	Coenzyme F420-dependent N(5),N(10)-methenyltetrahydromethanopterin reductase-related protein	polymer	370	41010.0	2	UniProt (F0S2B6) Pfam (PF22616) Pfam (PF03201) In PDB	Desulfurobacterium thermolithotrophum DSM 11699
3	A, C	iron-guanylyl pyridinol cofactor	non-polymer		686.3	2	Chemie (FE9)
4	A, D	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A, B, C, D	5,10-Methenyltetrahydrofolate	non-polymer		456.4	4	Chemie (GUE)
6	B, C, D	DIMETHYL SULFOXIDE	non-polymer		78.1	9	Chemie (DMS)
7	B	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
8		water	water		18.0	609	Chemie (HOH)

Sequence modifications

A, C: 1 - 357 (UniProt: F0S2B6)

PDB	External Database	Details
Lys 358	-	expression tag
Leu 359	-	expression tag
Ala 360	-	expression tag
Ala 361	-	expression tag
Ala 362	-	expression tag
Leu 363	-	expression tag
Glu 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

B, D: 1 - 357 (UniProt: F0S2B6)

PDB	External Database	Details
Lys 358	-	expression tag
Leu 359	-	expression tag
Ala 360	-	expression tag
Ala 361	-	expression tag
Ala 362	-	expression tag
Leu 363	-	expression tag
Glu 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	164008.0
Non-Polymers*	Number of molecules	18
	Total formula weight	4280.0
All*	Total formula weight	168288.0

*Water molecules are not included.