6HUT

GFP8 - a stabilized variant of cycle-3 GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	251	28405.0	1	UniProt (A0A059PIQ0) Pfam (PF01353) UniProt (by SIFTS) (P42212) In PDB	Aequorea victoria (Jellyfish)
2		water	water		18.0	211	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	Arg 2	engineered mutation
Arg 30	Ser 30	engineered mutation
Tyr 39	Asn 39	engineered mutation
Cro 66	Thr 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	engineered mutation
Arg 80	Gln 80	engineered mutation
Tyr 145	Phe 145	engineered mutation
Ile 171	Val 171	engineered mutation
Val 206	Ala 206	engineered mutation
Lys 239	Arg 239	engineered mutation
Leu 240	Pro 240	engineered mutation
Ala 243	Asn 243	engineered mutation
Leu 244	Asp 244	engineered mutation
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28405.0
All*	Total formula weight	28405.0

*Water molecules are not included.