6HUF

Coping with strong translational non-crystallographic symmetry and extreme anisotropy in molecular replacement with Phaser: human Rab27a

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, O, N, B, E...)	Ras-related protein Rab-27A	polymer	182	20761.4	16	UniProt (P51159) Pfam (PF00071)	Homo sapiens (Human)	Rab-27,GTP-binding protein Ram
2	AA, CA, EA, GA, IA... (C, K, M, D, P...)	MAGNESIUM ION	non-polymer		24.3	16	Chemie (MG)
3	BA, DA, FA, HA, JA... (C, K, M, D, P...)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	16	Chemie (GNP)
4	WA, XA (A, G)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, O, N, B, E, C, K, M, D, P, F, J, G, I, H, L: 2 - 182 (UniProt: P51159)

PDB	External Database	Details
Gly 1	-	expression tag
-	Arg 50	deletion
Thr 50	Val 51	conflict
Ile 51	Val 52	conflict
-	Ala 55	deletion
-	Ser 56	deletion
-	Gly 57	deletion
-	Pro 58	deletion
-	Asp 59	deletion
-	Gly 60	deletion
-	Ala 61	deletion
-	Thr 62	deletion
-	Gly 63	deletion
Asn 54	Arg 64	conflict
Asp 55	Gly 65	conflict
Lys 56	Gln 66	conflict
Lys 59	His 69	conflict
Leu 68	Gln 78	conflict
Glu 95	Gln 105	conflict
Lys 108	Gln 118	conflict
Thr 109	Met 119	conflict
Ser 113	Cys 123	conflict
Glu 130	Gln 140	conflict
Ala 134	Lys 144	conflict
Ala 135	Glu 145	conflict
Ala 136	Glu 146	conflict
Arg 139	Ile 149	conflict
Gln 140	Ala 150	conflict
His 144	Lys 154	conflict
Ser 178	Cys 188	conflict

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	332182.3
Non-Polymers*	Number of molecules	32
	Total formula weight	8744.0
All*	Total formula weight	340926.3

*Water molecules are not included.