6HUF
Coping with strong translational non-crystallographic symmetry and extreme anisotropy in molecular replacement with Phaser: human Rab27a
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, O, N, B, E... | Ras-related protein Rab-27A | polymer | 182 | 20761.4 | 16 | UniProt (P51159) Pfam (PF00071) In PDB | Homo sapiens (Human) | Rab-27,GTP-binding protein Ram |
| 2 | C, K, M, D, P... | MAGNESIUM ION | non-polymer | 24.3 | 16 | Chemie (MG) | |||
| 3 | C, K, M, D, P... | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 16 | Chemie (GNP) | |||
| 4 | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, O, N, B, E, C, K, M, D, P, F, J, G, I, H, L: 2 - 182 (UniProt: P51159)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| - | Arg 50 | deletion |
| Thr 50 | Val 51 | conflict |
| Ile 51 | Val 52 | conflict |
| - | Ala 55 | deletion |
| - | Ser 56 | deletion |
| - | Gly 57 | deletion |
| - | Pro 58 | deletion |
| - | Asp 59 | deletion |
| - | Gly 60 | deletion |
| - | Ala 61 | deletion |
| - | Thr 62 | deletion |
| - | Gly 63 | deletion |
| Asn 54 | Arg 64 | conflict |
| Asp 55 | Gly 65 | conflict |
| Lys 56 | Gln 66 | conflict |
| Lys 59 | His 69 | conflict |
| Leu 68 | Gln 78 | conflict |
| Glu 95 | Gln 105 | conflict |
| Lys 108 | Gln 118 | conflict |
| Thr 109 | Met 119 | conflict |
| Ser 113 | Cys 123 | conflict |
| Glu 130 | Gln 140 | conflict |
| Ala 134 | Lys 144 | conflict |
| Ala 135 | Glu 145 | conflict |
| Ala 136 | Glu 146 | conflict |
| Arg 139 | Ile 149 | conflict |
| Gln 140 | Ala 150 | conflict |
| His 144 | Lys 154 | conflict |
| Ser 178 | Cys 188 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 332182.3 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 8744.0 | |
| All* | Total formula weight | 340926.3 |
