6HR1

Crystal structure of the YFPnano fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Myosin light chain kinase 2, skeletal/cardiac muscle,Unconventional myosin-X,Green fluorescent protein,Calmodulin-1	polymer	436	49199.8	2	UniProt (P07313) UniProt (P79114) UniProt (P42212) UniProt (P0DP23) Pfam (PF01353) Pfam (PF13499)	Oryctolagus cuniculus (Rabbit)	MLCK2,Unconventional myosin-10
2	C (A)	L(+)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TLA)
3	D, E, F, G, M... (A, B)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
4	H, I, S, T, U... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
5	AA, J, K, L, Z (B, A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	Q, R (B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	BA, CA (A, B)	water	water		18.0	366	Chemie (HOH)

Sequence modifications

A, B: -30 - -11 (UniProt: P07313)

PDB	External Database	Details
Gly -35	-	expression tag
Pro -34	-	expression tag
His -33	-	expression tag
Met -32	-	expression tag
Ala -31	-	expression tag
Ala -26	Asn 584	engineered mutation

A, B: -10 - 0 (UniProt: P79114)
A, B: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Val 1	Met 1	conflict
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Leu 68	Val 68	conflict
Ala 72	Ser 72	conflict
Tyr 203	Thr 203	conflict
Leu 231	His 231	conflict
Gly 239	-	linker
Glu 240	-	linker
Asn 241	-	linker
Leu 242	-	linker
Tyr 243	-	linker
Phe 244	-	linker
Gln 245	-	linker
Ser 246	-	linker
Gly 247	-	linker
Gly 248	-	linker
Ser 249	-	linker
Ala 250	-	linker
Ala 251	-	linker
Ala 252	-	linker

A, B: 253 - 400 (UniProt: P0DP23)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98399.6
Non-Polymers*	Number of molecules	25
	Total formula weight	1535.8
All*	Total formula weight	99935.4

*Water molecules are not included.