6HR1

Crystal structure of the YFPnano fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	53.700, 117.800, 84.500
Unit cell angles	90.00, 99.90, 90.00

Refinement procedure

Resolution	48.589 - 1.901
R-factor	0.1823
Rwork	0.181
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v3d
RMSD bond length	0.009
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.589	48.589	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.090	0.043	0.954
Rmeas	0.097	0.047	1.062
Number of reflections	81149	940	5935
<I/σ(I)>	14	38.17	1.76
Completeness [%]	99.9	99.5	99.4
Redundancy	6.491	6.999	5.034
CC(1/2)	0.998	0.998	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	293	24% w/v PEG 3350 0.2 M di-Ammonium tartrate 10% v/v Glycerol