6HLM
Variant G129D of NuoEF from Aquifex aeolicus bound to NAD+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | NADH-quinone oxidoreductase subunit E | polymer | 160 | 18631.7 | 2 | UniProt (O66842) Pfam (PF01257) | Aquifex aeolicus | NADH dehydrogenase I subunit E,NDH-1 subunit E |
| 2 | B, D (B, D) | NADH-quinone oxidoreductase subunit F | polymer | 434 | 48523.3 | 2 | UniProt (O66841) Pfam (PF01512) Pfam (PF22461) Pfam (PF10531) Pfam (PF10589) | Aquifex aeolicus | NADH dehydrogenase I subunit F,NDH-1 subunit F |
| 3 | E, K (A, C) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 4 | F, L (B, D) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 5 | G, M (B, D) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 6 | H, N (B, D) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 7 | I (B) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 8 | J, O (B, D) | 3[N-MORPHOLINO]PROPANE SULFONIC ACID | non-polymer | 209.3 | 2 | Chemie (MPO) | |||
| 9 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 497 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 160 (UniProt: O66842)
B, D: 1 - 426 (UniProt: O66841)
| PDB | External Database | Details |
|---|---|---|
| Asp 129 | Gly 129 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 427 | - | expression tag |
| Gly 428 | - | expression tag |
| His 429 | - | expression tag |
| His 430 | - | expression tag |
| His 431 | - | expression tag |
| His 432 | - | expression tag |
| His 433 | - | expression tag |
| His 434 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 134309.9 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 3736.0 | |
| All* | Total formula weight | 138045.9 |
