Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6HIW

Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the complete small mitoribosomal subunit in complex with mt-IF-3

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	DA	mS48	polymer	1788	201811.2	1	UniProt (Q57UJ2) In PDB	Trypanosoma brucei brucei
2	DD	mS51	polymer	812	95081.0	1	UniProt (Q385L8) In PDB	Trypanosoma brucei brucei
3	DI	mS56	polymer	407	47306.8	1	UniProt (Q587C2) In PDB	Trypanosoma brucei brucei
4	DL	mS59	polymer	307	35395.7	1	UniProt (Q38BS2) In PDB	Trypanosoma brucei brucei
5	DM	mS60	polymer	294	34546.6	1	UniProt (Q57XL2) In PDB	Trypanosoma brucei brucei
6	DN	mS61	polymer	293	33482.3	1	UniProt (Q38D60) In PDB	Trypanosoma brucei brucei
7	DO	mS62	polymer	282	32068.1	1	UniProt (Q383D1) In PDB	Trypanosoma brucei brucei
8	DP	mS63	polymer	274	31922.2	1	UniProt (Q38F25) In PDB	Trypanosoma brucei brucei
9	DQ	mS64	polymer	268	30742.2	1	UniProt (Q382E8) Pfam (PF10469) In PDB	Trypanosoma brucei brucei
10	DR	mS65	polymer	270	30617.4	1	UniProt (Q57UA2) In PDB	Trypanosoma brucei brucei
11	DS	mS66	polymer	261	29960.2	1	UniProt (Q388L7) Pfam (PF14311) In PDB	Trypanosoma brucei brucei
12	DU	mS68	polymer	228	26472.8	1	UniProt (Q582T9) Pfam (PF00240) In PDB	Trypanosoma brucei brucei
13	DZ	mS73	polymer	94	11360.9	1	UniProt (Q587C4) In PDB	Trypanosoma brucei brucei
14	Da	mS74	polymer	64	8203.4	1		Trypanosoma brucei brucei
15	DB	mS49	polymer	1181	137234.1	1	UniProt (Q586P5) In PDB	Trypanosoma brucei brucei
16	DC	mS50	polymer	1165	131719.3	1	UniProt (Q57YB5) In PDB	Trypanosoma brucei brucei
17	DE	mS52	polymer	747	85012.3	1	UniProt (Q386Q7) In PDB	Trypanosoma brucei brucei
18	DF	mS53	polymer	666	75338.9	1	UniProt (Q38ET1) In PDB	Trypanosoma brucei brucei
19	DG	mS54	polymer	631	72046.0	1	UniProt (Q57ZP8) In PDB	Trypanosoma brucei brucei
20	DH	mS55	polymer	581	66676.7	1	UniProt (Q580V1) In PDB	Trypanosoma brucei brucei
21	DJ	mS57	polymer	396	45956.3	1	UniProt (Q584U8) In PDB	Trypanosoma brucei brucei
22	DK	mS58	polymer	324	35623.9	1	UniProt (Q38BP1) In PDB	Trypanosoma brucei brucei
23	DT	mS67	polymer	247	30096.1	1	UniProt (Q586G5) Pfam (PF00581) In PDB	Trypanosoma brucei brucei
24	DV	mS69	polymer	183	21589.7	1	UniProt (Q57UZ6) In PDB	Trypanosoma brucei brucei
25	DW	mS70	polymer	179	21247.4	1	UniProt (Q383N9) In PDB	Trypanosoma brucei brucei
26	DX	mS71	polymer	169	20093.2	1	UniProt (Q383G5) In PDB	Trypanosoma brucei brucei
27	DY	mS72	polymer	163	19317.3	1	UniProt (Q57YD4) In PDB	Trypanosoma brucei brucei
28	CC	uS3m	polymer	74	9070.9	1		Trypanosoma brucei brucei
29	CE	uS5m	polymer	435	50147.4	1	UniProt (Q38AX6) Pfam (PF03719) In PDB	Trypanosoma brucei brucei
30	CF	bS6m	polymer	160	18861.5	1	UniProt (Q38BW5) In PDB	Trypanosoma brucei brucei
31	CH	uS8m	polymer	282	32766.5	1	UniProt (Q388R7) Pfam (PF00410) In PDB	Trypanosoma brucei brucei
32	CI	uS9m	polymer	443	50201.0	1	UniProt (Q57W62) Pfam (PF00380) In PDB	Trypanosoma brucei brucei
33	CJ	uS10m	polymer	817	94351.0	1	UniProt (Q57Z45) Pfam (PF01476) In PDB	Trypanosoma brucei brucei
34	CK	uS11m	polymer	326	37925.9	1	UniProt (Q389T7) In PDB	Trypanosoma brucei brucei
35	CL	uS12m	polymer	87	10421.6	1		Trypanosoma brucei brucei
36	CN	uS14m	polymer	166	19900.0	1	UniProt (Q580I0) In PDB	Trypanosoma brucei brucei
37	CO	uS15m	polymer	429	50401.2	1	UniProt (Q4GZ99) In PDB	Trypanosoma brucei brucei
38	CP	bS16m	polymer	188	22077.8	1	UniProt (Q384N9) In PDB	Trypanosoma brucei brucei
39	CQ	uS17m	polymer	307	35632.7	1	UniProt (Q38DP8) Pfam (PF00366) In PDB	Trypanosoma brucei brucei
40	CR	bS18m	polymer	320	36988.7	1	UniProt (Q38AS2) In PDB	Trypanosoma brucei brucei
41	CS	uS19m	polymer	244	28364.6	1	UniProt (Q584T8) In PDB	Trypanosoma brucei brucei
42	CU	bS21m	polymer	193	23087.9	1	UniProt (Q580M9) In PDB	Trypanosoma brucei brucei
43	CZ	mt-IF-3	polymer	360	41086.3	1	UniProt (Q57WU2) In PDB	Trypanosoma brucei brucei
44	Ca	mS22	polymer	602	73064.1	1	UniProt (Q38DR3) In PDB	Trypanosoma brucei brucei
45	Cb	mS23	polymer	325	36855.6	1	UniProt (Q57VB2) In PDB	Trypanosoma brucei brucei
46	Cd	mS26	polymer	440	51164.7	1	UniProt (Q38DK6) In PDB	Trypanosoma brucei brucei
47	Cg	mS29	polymer	498	57048.8	1	UniProt (Q585C2) Pfam (PF10236) In PDB	Trypanosoma brucei brucei
48	Ci	mS33	polymer	181	21244.1	1	UniProt (Q57WW0) In PDB	Trypanosoma brucei brucei
49	Cj	mS34	polymer	257	28728.2	1	UniProt (Q57UK0) In PDB	Trypanosoma brucei brucei
50	Ck	mS35	polymer	874	98377.8	1	UniProt (Q387C7) In PDB	Trypanosoma brucei brucei
51	Cm	mS37	polymer	215	24757.9	1	UniProt (Q38C96) In PDB	Trypanosoma brucei brucei
52	Cn	mS38	polymer	250	28240.6	1	UniProt (Q57VQ9) In PDB	Trypanosoma brucei brucei
53	Cp	mS41	polymer	187	22421.5	1	UniProt (Q389L3) In PDB	Trypanosoma brucei brucei
54	Cq	mS42	polymer	263	29748.7	1	UniProt (Q586A1) Pfam (PF02777) In PDB	Trypanosoma brucei brucei
55	Cr	mS43	polymer	439	48590.4	1	UniProt (Q585I1) Pfam (PF02777) In PDB	Trypanosoma brucei brucei
56	Cv	mS47	polymer	1211	137929.0	1	UniProt (Q383R4) Pfam (PF16113) In PDB	Trypanosoma brucei brucei
57	CA	9S rRNA	polymer	621	198442.4	1	GenBank (343546)	Trypanosoma brucei brucei
58	UO	Unknown Protein	polymer	5	443.5	1		Trypanosoma brucei brucei
59	UP	Unknown Protein	polymer	7	613.7	1		Trypanosoma brucei brucei
60	UQ	Unknown Protein	polymer	32	2741.4	1		Trypanosoma brucei brucei
61	UR	Unknown Protein	polymer	8	698.9	1		Trypanosoma brucei brucei
62	US	Unknown Protein	polymer	54	4613.7	1		Trypanosoma brucei brucei
63	UT	Unknown Protein	polymer	44	3762.6	1		Trypanosoma brucei brucei
64	DA, DS, Cr	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
65	DJ	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	1	Chemie (UTP)
66	CA, CQ, Ca, Cg, Cv	MAGNESIUM ION	non-polymer		24.3	39	Chemie (MG)
67	Cg	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
68	CA	SPERMIDINE	non-polymer		145.2	4	Chemie (SPD)
69	CA	SPERMINE	non-polymer		202.3	1	Chemie (SPM)
70		water	water		18.0	3	Chemie (HOH)

Sequence modifications

DA: 1 - 1788 (UniProt: Q57UJ2)

PDB	External Database	Details
His 894	Asn 894	conflict
Thr 1181	Ile 1181	conflict
Ala 1333	Val 1333	conflict
Arg 1700	His 1700	conflict
Lys 1761	Arg 1761	conflict

DD: 1 - 812 (UniProt: Q385L8)

PDB	External Database	Details
Pro 371	Ser 371	conflict
Ala 599	Val 599	conflict

DI: 1 - 407 (UniProt: Q587C2)

PDB	External Database	Details
Glu 92	Gly 92	conflict
Asp 116	Glu 116	conflict

DL: 1 - 307 (UniProt: Q38BS2)

PDB	External Database	Details
Thr 274	Ala 274	conflict

DM: 1 - 294 (UniProt: Q57XL2)

PDB	External Database	Details
Phe 69	Tyr 69	conflict
Asn 97	Ser 97	conflict
Ser 138	Pro 138	conflict
Ala 173	Thr 173	conflict
Ala 206	Thr 206	conflict

DN: 1 - 293 (UniProt: Q38D60)

PDB	External Database	Details
Gly 51	Ser 51	conflict

DP: 1 - 274 (UniProt: Q38F25)

PDB	External Database	Details
His 3	Arg 3	conflict

DR: 1 - 270 (UniProt: Q57UA2)

PDB	External Database	Details
Gly 65	Ser 65	conflict
Gly 94	Glu 94	conflict
Pro 128	Ser 128	conflict
Arg 229	Gln 229	conflict

DU: 1 - 228 (UniProt: Q582T9)

PDB	External Database	Details
Ile 119	Leu 119	conflict
Ile 152	Val 152	conflict

DB: 1 - 1181 (UniProt: Q586P5)

PDB	External Database	Details
Val 23	Ala 23	conflict
Ile 359	Thr 359	conflict
Gln 384	His 384	conflict
Thr 402	Ile 402	conflict
Thr 423	Ala 423	conflict
Arg 586	His 586	conflict
Arg 593	Lys 593	conflict
Ser 647	Gly 647	conflict

DC: 1 - 1165 (UniProt: Q57YB5)

PDB	External Database	Details
Ala 53	Thr 53	conflict
Lys 365	Glu 365	conflict
Thr 385	Ala 385	conflict
Ile 405	Val 405	conflict
Ser 641	Pro 641	conflict
Lys 651	Glu 651	conflict
Glu 731	Asp 731	conflict
Gln 814	His 814	conflict
Ala 1097	Val 1097	conflict
Thr 1113	Ile 1113	conflict

DE: 1 - 747 (UniProt: Q386Q7)

PDB	External Database	Details
Thr 514	Ser 514	conflict

DF: 1 - 666 (UniProt: Q38ET1)

PDB	External Database	Details
Thr 18	Ala 18	conflict
Asp 258	Asn 258	conflict
Asn 372	Asp 372	conflict
Asn 406	Ser 406	conflict
Asp 510	Gly 510	conflict
Ala 577	Val 577	conflict
Unk 636	Gly 636	conflict
Lys 638	Arg 638	conflict

DG: 1 - 631 (UniProt: Q57ZP8)

PDB	External Database	Details
Asn 428	Ser 428	conflict
Gly 429	Ser 429	conflict

DH: 1 - 581 (UniProt: Q580V1)

PDB	External Database	Details
His 191	Gln 191	conflict
Pro 194	Arg 194	conflict
Gly 488	Ser 488	conflict

DK: 1 - 324 (UniProt: Q38BP1)

PDB	External Database	Details
Ser 61	Pro 131	conflict
Gly 257	Ser 327	conflict

DV: 1 - 183 (UniProt: Q57UZ6)

PDB	External Database	Details
Ala 163	Thr 163	conflict

DW: 1 - 179 (UniProt: Q383N9)

PDB	External Database	Details
Thr 74	Met 74	conflict

CE: 1 - 435 (UniProt: Q38AX6)

PDB	External Database	Details
Arg 341	Lys 341	conflict

CH: 1 - 282 (UniProt: Q388R7)

PDB	External Database	Details
Asn 74	Ser 74	conflict

CI: 1 - 443 (UniProt: Q57W62)

PDB	External Database	Details
Ala 370	Val 370	conflict

CJ: 1 - 817 (UniProt: Q57Z45)

PDB	External Database	Details
Leu 311	Tyr 311	conflict
His 484	Arg 484	conflict
Ser 488	Asn 488	conflict
Glu 594	Val 594	conflict
Arg 629	Lys 629	conflict

CK: 1 - 326 (UniProt: Q389T7)

PDB	External Database	Details
Arg 3	Gln 3	conflict
Unk 138	Ile 138	conflict

CQ: 1 - 307 (UniProt: Q38DP8)

PDB	External Database	Details
Ala 138	Val 138	conflict

CR: 1 - 320 (UniProt: Q38AS2)

PDB	External Database	Details
Ile 8	Val 8	conflict

CS: 1 - 172 (UniProt: Q584T8)

PDB	External Database	Details
Met -71	-	initiating methionine
Ala -70	-	expression tag
Phe -69	-	expression tag
Arg -68	-	expression tag
Asn -67	-	expression tag
Thr -66	-	expression tag
Phe -65	-	expression tag
Thr -64	-	expression tag
Thr -63	-	expression tag
Pro -62	-	expression tag
Gly -61	-	expression tag
Lys -60	-	expression tag
Phe -59	-	expression tag
Ser -58	-	expression tag
Thr -57	-	expression tag
Val -56	-	expression tag
Ser -55	-	expression tag
Lys -54	-	expression tag
Asn -53	-	expression tag
Ile -52	-	expression tag
Val -51	-	expression tag
Leu -50	-	expression tag
Leu -49	-	expression tag
Leu -48	-	expression tag
Ile -47	-	expression tag
Trp -46	-	expression tag
Arg -45	-	expression tag
Val -44	-	expression tag
Lys -43	-	expression tag
Val -42	-	expression tag
Phe -41	-	expression tag
Leu -40	-	expression tag
Arg -39	-	expression tag
Ala -38	-	expression tag
Glu -37	-	expression tag
Gly -36	-	expression tag
Phe -35	-	expression tag
Ala -34	-	expression tag
His -33	-	expression tag
Ser -32	-	expression tag
Leu -31	-	expression tag
Val -30	-	expression tag
Met -29	-	expression tag
Leu -28	-	expression tag
Pro -27	-	expression tag
Val -26	-	expression tag
Ser -25	-	expression tag
Leu -24	-	expression tag
Tyr -23	-	expression tag
Ser -22	-	expression tag
Lys -21	-	expression tag
Ile -20	-	expression tag
Leu -19	-	expression tag
Leu -18	-	expression tag
Cys -17	-	expression tag
Asp -16	-	expression tag
Val -15	-	expression tag
Lys -14	-	expression tag
Lys -13	-	expression tag
Lys -12	-	expression tag
Ile -11	-	expression tag
Val -10	-	expression tag
Tyr -9	-	expression tag
Phe -8	-	expression tag
His -7	-	expression tag
Cys -6	-	expression tag
Cys -5	-	expression tag
Thr -4	-	expression tag
Arg -3	-	expression tag
Lys -2	-	expression tag
Lys -1	-	expression tag
Ser 0	-	expression tag

CZ: 1 - 360 (UniProt: Q57WU2)

PDB	External Database	Details
Ser 6	Gly 6	conflict
Thr 30	Ile 30	conflict
Thr 172	Ala 172	conflict

Cb: 1 - 311 (UniProt: Q57VB2)

PDB	External Database	Details
Ser 244	Asn 244	conflict
-	Glu 246	deletion
Ala 312	-	expression tag
Cys 313	-	expression tag
Ser 314	-	expression tag
Arg 315	-	expression tag
Asp 316	-	expression tag
Gly 317	-	expression tag
Phe 318	-	expression tag
Ala 319	-	expression tag
Leu 320	-	expression tag
Met 321	-	expression tag
Lys 322	-	expression tag
Ala 323	-	expression tag
Asn 324	-	expression tag
Lys 325	-	expression tag

Cd: 1 - 440 (UniProt: Q38DK6)

PDB	External Database	Details
Unk 299	Gly 299	conflict

Cg: 1 - 497 (UniProt: Q585C2)

PDB	External Database	Details
Val 181	Ala 181	conflict
Arg 498	-	expression tag

Ck: 1 - 874 (UniProt: Q387C7)

PDB	External Database	Details
Ser 107	Leu 107	conflict
Phe 144	Leu 144	conflict
Tyr 253	Phe 253	conflict
Glu 339	Val 339	conflict
Gly 871	Glu 871	conflict

Cq: 1 - 263 (UniProt: Q586A1)

PDB	External Database	Details
Thr 48	Ala 48	conflict
Met 167	Val 167	conflict

Cr: 1 - 439 (UniProt: Q585I1)

PDB	External Database	Details
Lys 351	Glu 351	conflict

Cv: 1 - 1211 (UniProt: Q383R4)

PDB	External Database	Details
Thr 718	Ala 718	conflict
Glu 1179	Gly 1179	conflict

CA: 1 - 613 (GenBank: 343546)

PDB	External Database	Details
U 298	C 2839	conflict
U 473	G 3014	conflict
U 614	-	insertion
U 615	-	insertion
U 616	-	insertion
U 617	-	insertion
U 618	-	insertion
U 619	-	insertion
U 620	-	insertion
U 621	-	insertion

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	63
Polymers	Total formula weight	2841694.7
Non-Polymers*	Number of molecules	50
Non-Polymers*	Total formula weight	3000.2
All*	Total formula weight	2844694.8

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)