6HCO

Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ATP-binding cassette sub-family G member 2	polymer	664	73394.8	2	UniProt (Q9UNQ0) Pfam (PF00005) Pfam (PF19055) Pfam (PF01061) In PDB	Homo sapiens (Human)	Breast cancer resistance protein,CDw338,Mitoxantrone resistance-associated protein,Placenta-specific ATP-binding cassette transporter,Urate exporter
2	C, E	5D3-Fab light chain	polymer	214	23594.0	2		Mus musculus (House Mouse)
3	D, F	5D3-Fab heavy chain	polymer	221	23843.6	2		Mus musculus (House Mouse)
4	G, H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	A	estrone 3-sulfate	non-polymer		350.4	1	Chemie (FY5)

Sequence modifications

A, B: 2 - 655 (UniProt: Q9UNQ0)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gln 211	Glu 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	241664.8
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	1
	Total formula weight	350.4
All*	Total formula weight	242864.0

*Water molecules are not included.