6HCO

Cryo-EM structure of the ABCG2 E211Q mutant bound to estrone 3-sulfate and 5D3-Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, B)	ATP-binding cassette sub-family G member 2	polymer	664	73394.8	2	UniProt (Q9UNQ0) Pfam (PF00005) Pfam (PF19055) Pfam (PF01061)	Homo sapiens (Human)	Breast cancer resistance protein,CDw338,Mitoxantrone resistance-associated protein,Placenta-specific ATP-binding cassette transporter,Urate exporter
2	B, D (C, E)	5D3-Fab light chain	polymer	214	23594.0	2		Mus musculus (House Mouse)
3	C, E (D, F)	5D3-Fab heavy chain	polymer	221	23843.6	2		Mus musculus (House Mouse)
4	G, H (G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	I (A)	estrone 3-sulfate	non-polymer		350.4	1	Chemie (FY5) PubChem (699) PubChem (5458404) PubChem (6093179) PubChem (6604331) PubChem (10861069) PubChem (13210801) PubChem (40467170) PubChem (3032531) PubChem (69814994) PubChem (3001028) PubChem (86308130) PubChem (5281105) PubChem (56979030) PubChem (92209271) PubChem (122173120) PubChem (124303481) PubChem (124540992) PubChem (133121260) PubChem (133688320)

Sequence modifications

A, B: 2 - 655 (UniProt: Q9UNQ0)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gln 211	Glu 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	241664.8
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	1
	Total formula weight	350.4
All*	Total formula weight	242864.0

*Water molecules are not included.