6H8N

Structure of peptidoglycan deacetylase PdaC from Bacillus subtilis - mutant D285S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Peptidoglycan-N-acetylmuramic acid deacetylase PdaC	polymer	212	24486.7	2	UniProt (O34798) Pfam (PF01522) In PDB	Bacillus subtilis subsp. subtilis str. 168	Peptidoglycan MurNAc deacetylase,Polysaccharide deacetylase PdaC
2	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	A, B	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	467	Chemie (HOH)

Sequence modifications

A, B: 245 - 442 (UniProt: O34798)

PDB	External Database	Details
Met 244	-	initiating methionine
Ser 260	Asp 285	engineered mutation
Glu 443	-	expression tag
Leu 444	-	expression tag
Arg 445	-	expression tag
Arg 446	-	expression tag
Gln 447	-	expression tag
Trp 448	-	expression tag
Ser 449	-	expression tag
His 450	-	expression tag
Pro 451	-	expression tag
Gln 452	-	expression tag
Phe 453	-	expression tag
Glu 454	-	expression tag
Lys 455	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48973.5
Non-Polymers*	Number of molecules	7
	Total formula weight	599.9
All*	Total formula weight	49573.4

*Water molecules are not included.