6H2A

Structure of S70A BlaC from Mycobacterium tuberculosis obtained from crystals produced in the presence of DTT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Beta-lactamase	polymer	274	29459.1	3	UniProt (A0A0T9EA39)	Mycobacterium tuberculosis
2	D (H)	Possible protein degradation product	polymer	6	360.3	1		Mycobacterium tuberculosis
3	E, J (A)	TRIS(HYDROXYETHYL)AMINOMETHANE	non-polymer		163.2	2	Chemie (TAM)
4	F, L, M, R (A, B, C)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	4	Chemie (DTT)
5	G, H, I, N, O... (A, B, C)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
6	K, P, Q, T (A, B, C)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7	U, V, W, X (A, B, C, H)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B, C: 29 - 293 (UniProt: A0A0T9EA39)

PDB	External Database	Details
Met 28	-	initiating methionine
Ala 70	Ser 47	engineered mutation
Leu 294	-	expression tag
Glu 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88737.5
Non-Polymers*	Number of molecules	16
	Total formula weight	1881.6
All*	Total formula weight	90619.1

*Water molecules are not included.