6H25

Human nuclear RNA exosome EXO-10-MPP6 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Exosome complex component RRP45	polymer	443	49370.3	1	UniProt (Q06265) Pfam (PF01138) Pfam (PF03725)	Homo sapiens (Human)	Autoantigen PM/Scl 1,Exosome component 9,P75 polymyositis-scleroderma overlap syndrome-associated autoantigen,Polymyositis/scleroderma autoantigen 1,Polymyositis/scleroderma autoantigen 75 kDa,PM/Scl-75
2	B (B)	Exosome complex component RRP41	polymer	249	26773.3	1	UniProt (Q9NPD3) Pfam (PF01138) Pfam (PF03725)	Homo sapiens (Human)	Exosome component 4,Ribosomal RNA-processing protein 41,p12A
3	C (C)	Exosome complex component RRP43	polymer	278	30317.8	1	UniProt (Q96B26) Pfam (PF01138) Pfam (PF03725)	Homo sapiens (Human)	Exosome component 8,Opa-interacting protein 2,OIP-2,Ribosomal RNA-processing protein 43,p9
4	D (D)	Exosome complex component RRP46	polymer	237	25524.3	1	UniProt (Q9NQT4) Pfam (PF01138) Pfam (PF03725)	Homo sapiens (Human)	Chronic myelogenous leukemia tumor antigen 28,Exosome component 5,Ribosomal RNA-processing protein 46,p12B
5	E (E)	Exosome complex component RRP42	polymer	295	32216.8	1	UniProt (Q15024) Pfam (PF03725) Pfam (PF01138)	Homo sapiens (Human)	Exosome component 7,Ribosomal RNA-processing protein 42,p8
6	F (F)	Exosome complex component MTR3	polymer	276	28623.5	1	UniProt (Q5RKV6) Pfam (PF01138)	Homo sapiens (Human)	Exosome component 6,mRNA transport regulator 3 homolog,hMtr3,p11
7	G (G)	Exosome complex component RRP40	polymer	293	30906.4	1	UniProt (Q9NQT5) Pfam (PF21261) Pfam (PF21262) Pfam (PF15985)	Homo sapiens (Human)	Exosome component 3,Ribosomal RNA-processing protein 40,p10
8	H (H)	Exosome complex component RRP4	polymer	297	33190.4	1	UniProt (Q13868) Pfam (PF14382) Pfam (PF21266) Pfam (PF15985)	Homo sapiens (Human)	Exosome component 2,Ribosomal RNA-processing protein 4
9	I (I)	Exosome complex component CSL4	polymer	199	21835.1	1	UniProt (Q9Y3B2) Pfam (PF14382) Pfam (PF10447)	Homo sapiens (Human)	Exosome component 1
10	J (J)	Exosome complex exonuclease RRP44	polymer	962	109522.2	1	UniProt (Q9Y2L1) Pfam (PF13638) Pfam (PF17216) Pfam (PF17849) Pfam (PF00773) Pfam (PF17215)	Homo sapiens (Human)	Protein DIS3 homolog,Ribosomal RNA-processing protein 44
11	K (K)	M-phase phosphoprotein 6	polymer	166	19542.2	1	UniProt (Q99547) Pfam (PF10175)	Homo sapiens (Human)
12	L (R)	U44 ssRNA	polymer	44	13426.3	1		synthetic construct

Sequence modifications

A: 1 - 439 (UniProt: Q06265)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 245 (UniProt: Q9NPD3)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 276 (UniProt: Q96B26)

PDB	External Database	Details
Arg -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 235 (UniProt: Q9NQT4)

PDB	External Database	Details
Arg -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 291 (UniProt: Q15024)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

F: 1 - 272 (UniProt: Q5RKV6)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

G: 1 - 275 (UniProt: Q9NQT5)

PDB	External Database	Details
Gly -17	-	expression tag
Ser -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

H: 1 - 293 (UniProt: Q13868)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

I: 1 - 195 (UniProt: Q9Y3B2)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

J: 1 - 958 (UniProt: Q9Y2L1)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag
Asn 146	Asp 146	engineered mutation
Asn 487	Asp 487	engineered mutation

K: 1 - 160 (UniProt: Q99547)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Asp -3	-	expression tag
Ser -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Lys 8	Arg 8	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	421248.6
All*	Total formula weight	421248.6

*Water molecules are not included.