6H01
Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Green fluorescent protein | polymer | 266 | 29908.4 | 4 | UniProt (A0A059PIQ0) Pfam (PF01353) In PDB | Aequorea victoria (Jellyfish) | |
2 | water | water | 18.0 | 133 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 3 - 238 (UniProt: A0A059PIQ0)
PDB | External Database | Details |
---|---|---|
Met -12 | - | initiating methionine |
Gly -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Val 1 | - | expression tag |
Ser 2 | - | expression tag |
Arg 30 | Ser 30 | conflict |
Fhe 66 | Thr 65 | chromophore |
Fhe 66 | Tyr 66 | chromophore |
Fhe 66 | Gly 67 | chromophore |
Ser 72 | Ala 72 | conflict |
Lys 206 | Ala 206 | conflict |
Leu 231 | His 231 | conflict |
Gly 239 | - | expression tag |
Ile 240 | - | expression tag |
Glu 241 | - | expression tag |
Glu 242 | - | expression tag |
Asn 243 | - | expression tag |
Leu 244 | - | expression tag |
Tyr 245 | - | expression tag |
Phe 246 | - | expression tag |
Gln 247 | - | expression tag |
Ser 248 | - | expression tag |
Asn 249 | - | expression tag |
Ile 250 | - | expression tag |
Gly 251 | - | expression tag |
Ser 252 | - | expression tag |
Gly 253 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 119633.6 | |
All* | Total formula weight | 119633.6 |