6H01

Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green fluorescent protein	polymer	266	29908.4	4	UniProt (A0A059PIQ0) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	E, F, G, H (A, B, C, D)	water	water		18.0	133	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 238 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Val 1	-	expression tag
Ser 2	-	expression tag
Arg 30	Ser 30	conflict
Fhe 66	Thr 65	chromophore
Fhe 66	Tyr 66	chromophore
Fhe 66	Gly 67	chromophore
Ser 72	Ala 72	conflict
Lys 206	Ala 206	conflict
Leu 231	His 231	conflict
Gly 239	-	expression tag
Ile 240	-	expression tag
Glu 241	-	expression tag
Glu 242	-	expression tag
Asn 243	-	expression tag
Leu 244	-	expression tag
Tyr 245	-	expression tag
Phe 246	-	expression tag
Gln 247	-	expression tag
Ser 248	-	expression tag
Asn 249	-	expression tag
Ile 250	-	expression tag
Gly 251	-	expression tag
Ser 252	-	expression tag
Gly 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119633.6
All*	Total formula weight	119633.6

*Water molecules are not included.