6H01
Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2018-04-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 155.324, 155.324, 161.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.317 - 2.776 |
R-factor | 0.2027 |
Rwork | 0.201 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ema |
RMSD bond length | 0.009 |
RMSD bond angle | 1.074 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.930 | 3.338 |
High resolution limit [Å] | 2.776 | 2.776 |
Rmerge | 0.055 | 0.609 |
Number of reflections | 54903 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 100.0 | |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.1 M tri-Na-citrate 0.1 M Na-HEPES |