6GYP

Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Centromere DNA-binding protein complex CBF3 subunit B	polymer	608	71439.9	1	UniProt (P40969) Pfam (PF00172) Pfam (PF16846) In PDB	Saccharomyces cerevisiae S288C	Centromere protein 3
2	A	Centromere DNA-binding protein complex CBF3 subunit C	polymer	478	56416.9	1	UniProt (P35203) In PDB	Saccharomyces cerevisiae S288C	Chromosome transmission fidelity protein 13,Kinetochore protein CTF13
3	C	Centromere DNA-binding protein complex CBF3 subunit B	polymer	564	66401.9	1	UniProt (P40969) Pfam (PF16846) In PDB	Saccharomyces cerevisiae S288C	Centromere protein 3
4	D	Suppressor of kinetochore protein 1	polymer	194	22357.3	1	UniProt (P52286) Pfam (PF03931) Pfam (PF01466) In PDB	Saccharomyces cerevisiae S288C	Centromere DNA-binding protein complex CBF3 subunit D,E3 ubiquitin ligase complex SCF subunit SKP1
5	E	Centromere DNA-binding protein complex CBF3 subunit A	polymer	956	112066.0	1	UniProt (P32504) Pfam (PF21400) Pfam (PF16787) Pfam (PF12550) In PDB	Saccharomyces cerevisiae S288C	Centromere-binding factor 2,Chromosome transmission fidelity protein 14,Kinetochore protein CTF14
6	B	METHIONINE	non-polymer		149.2	1	Chemie (MET)
7	B	PHENYLALANINE	non-polymer		165.2	1	Chemie (PHE)
8	B	ASPARAGINE	non-polymer		132.1	1	Chemie (ASN)
9	B	ARGININE	non-polymer		175.2	1	Chemie (ARG)
10	B	THREONINE	non-polymer		119.1	2	Chemie (THR)
11	B	GLUTAMINE	non-polymer		146.1	1	Chemie (GLN)

Sequence modifications

C: 49 - 608 (UniProt: P40969)

PDB	External Database	Details
Met 1	-	initiating methionine
Phe 2	-	expression tag
Asn 3	-	expression tag
Arg 4	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	328681.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1006.1
All*	Total formula weight	329688.0

*Water molecules are not included.