Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6GYP

Cryo-EM structure of the CBF3-core-Ndc10-DBD complex of the budding yeast kinetochore

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000921	biological_process	septin ring assembly
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0008301	molecular_function	DNA binding, bending
A	0031518	cellular_component	CBF3 complex
A	0051382	biological_process	kinetochore assembly
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000921	biological_process	septin ring assembly
B	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0006355	biological_process	regulation of DNA-templated transcription
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0008270	molecular_function	zinc ion binding
B	0008301	molecular_function	DNA binding, bending
B	0019237	molecular_function	centromeric DNA binding
B	0031518	cellular_component	CBF3 complex
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0051382	biological_process	kinetochore assembly
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000921	biological_process	septin ring assembly
C	0003677	molecular_function	DNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0008270	molecular_function	zinc ion binding
C	0008301	molecular_function	DNA binding, bending
C	0019237	molecular_function	centromeric DNA binding
C	0031518	cellular_component	CBF3 complex
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0051382	biological_process	kinetochore assembly
D	0000082	biological_process	G1/S transition of mitotic cell cycle
D	0000086	biological_process	G2/M transition of mitotic cell cycle
D	0000278	biological_process	mitotic cell cycle
D	0000409	biological_process	regulation of transcription by galactose
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000781	cellular_component	chromosome, telomeric region
D	0000921	biological_process	septin ring assembly
D	0003677	molecular_function	DNA binding
D	0003688	molecular_function	DNA replication origin binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0007035	biological_process	vacuolar acidification
D	0007096	biological_process	regulation of exit from mitosis
D	0007346	biological_process	regulation of mitotic cell cycle
D	0008053	biological_process	mitochondrial fusion
D	0010458	biological_process	exit from mitosis
D	0010828	biological_process	positive regulation of glucose transmembrane transport
D	0012505	cellular_component	endomembrane system
D	0016567	biological_process	protein ubiquitination
D	0019005	cellular_component	SCF ubiquitin ligase complex
D	0019222	biological_process	regulation of metabolic process
D	0030466	biological_process	silent mating-type cassette heterochromatin formation
D	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
D	0031335	biological_process	regulation of sulfur amino acid metabolic process
D	0031509	biological_process	subtelomeric heterochromatin formation
D	0031518	cellular_component	CBF3 complex
D	0031573	biological_process	mitotic intra-S DNA damage checkpoint signaling
D	0043254	biological_process	regulation of protein-containing complex assembly
D	0043291	cellular_component	RAVE complex
D	0045116	biological_process	protein neddylation
D	0051382	biological_process	kinetochore assembly
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0065003	biological_process	protein-containing complex assembly
D	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
D	0071406	biological_process	cellular response to methylmercury
D	0097602	molecular_function	cullin family protein binding
D	2000766	biological_process	negative regulation of cytoplasmic translation
E	0000022	biological_process	mitotic spindle elongation
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000779	cellular_component	condensed chromosome, centromeric region
E	0000921	biological_process	septin ring assembly
E	0003677	molecular_function	DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005816	cellular_component	spindle pole body
E	0005819	cellular_component	spindle
E	0005856	cellular_component	cytoskeleton
E	0007059	biological_process	chromosome segregation
E	0008301	molecular_function	DNA binding, bending
E	0019237	molecular_function	centromeric DNA binding
E	0031518	cellular_component	CBF3 complex
E	0051233	cellular_component	spindle midzone
E	0051382	biological_process	kinetochore assembly

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue MET B 701

Chain	Residue
B	PHE64
B	GLN542
B	PHE702

site_id	AC2
Number of Residues	4
Details	binding site for residue PHE B 702

Chain	Residue
B	SER532
B	PHE533
B	MET701
B	ASN703

site_id	AC3
Number of Residues	5
Details	binding site for residue ASN B 703

Chain	Residue
B	PHE702
B	ARG704
B	THR705
B	ASP60
B	ASP531

site_id	AC4
Number of Residues	6
Details	binding site for residue ARG B 704

Chain	Residue
B	GLU56
B	GLY530
B	ASP531
B	ASN703
B	THR705
B	THR706

site_id	AC5
Number of Residues	5
Details	binding site for residue THR B 705

Chain	Residue
B	GLU56
B	ASP60
B	ASN703
B	ARG704
B	THR706

site_id	AC6
Number of Residues	4
Details	binding site for residue THR B 706

Chain	Residue
B	GLU56
B	ARG704
B	THR705
B	GLN707

site_id	AC7
Number of Residues	1
Details	binding site for residue GLN B 707

Chain	Residue
B	THR706

Functional Information from PROSITE/UniProt

site_id	PS00463
Number of Residues	30
Details	ZN2_CY6_FUNGAL_1 Zn(2)-C6 fungal-type DNA-binding domain signature. PCsvCTrrKvkCdrmip.......CgnCrkrgqdseC

Chain	Residue	Details
B	PRO13-CYS42

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
E	SER566

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
E	TYR706

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)