6GXC

Bacterial oligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Undecaprenyl-diphosphooligosaccharide--protein glycotransferase	polymer	724	84566.0	1	UniProt (B9KDD4) Pfam (PF02516) Pfam (PF21436) Pfam (PF18527) In PDB	Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)	Protein glycosylation B
2	B	GLY-ASP-GLN-DAB-ALA-THR-PPN-GLY	polymer	8	839.8	1		Campylobacter lari RM2100
3	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	A	[(2~{R},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl] [oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl] hydrogen phosphate	non-polymer		653.6	1	Chemie (FFK)

Sequence modifications

A: 1 - 712 (UniProt: B9KDD4)

PDB	External Database	Details
Glu 2	Lys 2	engineered mutation
Ala 17	Cys 17	engineered mutation
Ala 30	Cys 30	engineered mutation
Thr 108	Ala 108	engineered mutation
Leu 350	Cys 350	engineered mutation
Gln 535	Asn 535	engineered mutation
Pro 549	Lys 549	engineered mutation
Asn 550	Asp 550	engineered mutation
Ile 553	Phe 553	engineered mutation
Pro 556	Asn 556	engineered mutation
Pro 600	Ala 600	engineered mutation
Leu 601	Ile 601	engineered mutation
Asp 602	Ala 602	engineered mutation
Lys 606	Thr 606	engineered mutation
Gln 607	Thr 607	engineered mutation
Ile 610	Val 610	engineered mutation
Thr 611	Met 611	engineered mutation
Ser 619	Ile 619	engineered mutation
Tyr 622	Phe 622	engineered mutation
Ser 624	Ala 624	engineered mutation
Ile 627	Val 627	engineered mutation
Asn 630	Ala 630	engineered mutation
Tyr 663	Phe 663	engineered mutation
Tyr 670	Phe 670	engineered mutation
Glu 713	-	expression tag
Phe 714	-	expression tag
His 715	-	expression tag
His 716	-	expression tag
His 717	-	expression tag
His 718	-	expression tag
His 719	-	expression tag
His 720	-	expression tag
His 721	-	expression tag
His 722	-	expression tag
His 723	-	expression tag
His 724	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85405.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1087.9
All*	Total formula weight	86493.6

*Water molecules are not included.