6GXC
Bacterial oligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.810, 116.540, 173.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.094 - 3.401 |
R-factor | 0.2522 |
Rwork | 0.249 |
R-free | 0.28310 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.157 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (v1.12) |
Refinement software | PHENIX (v1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.094 | 3.490 |
High resolution limit [Å] | 3.401 | 3.401 |
Rmeas | 0.069 | 1.440 |
Number of reflections | 24002 | |
<I/σ(I)> | 23.28 | |
Completeness [%] | 92.1 | 33.7 |
Redundancy | 13.4 | 4.8 |
CC(1/2) | 0.999 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 293 | 0.1M glycine, pH 9.4, 0.15M magnesium acetate, and 30% PEG400 |