6GWG

Alpha-galactosidase from Thermotoga maritima in complex with cyclohexene-based carbasugar mimic of galactose covalently linked to the nucleophile

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-galactosidase	polymer	575	66198.1	1	UniProt (G4FEF4) Pfam (PF22676) Pfam (PF02065) In PDB	Thermotoga maritima MSB8	Melibiase
2	A	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
3	A	(1~{S},2~{S},3~{S})-3-fluoranyl-6-(hydroxymethyl)cyclohex-5-ene-1,2,4-triol	non-polymer		178.2	1	Chemie (FEK)
4	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	470	Chemie (HOH)

Sequence modifications

A: 1 - 552 (UniProt: G4FEF4)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	66198.1
Non-Polymers*	Number of molecules	14
	Total formula weight	1203.8
All*	Total formula weight	67401.9

*Water molecules are not included.