6GVS

Engineered glycolyl-CoA reductase comprising 8 mutations with bound NADP+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Aldehyde dehydrogenase	polymer	522	55275.1	10	UniProt (Q21A49) Pfam (PF00171) In PDB	Rhodopseudomonas palustris BisB18
2	I, J, A, B, C...	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	10	Chemie (NAP)	Rhodopseudomonas palustris BisB18
3	I, J, A, B, C...	POTASSIUM ION	non-polymer		39.1	10	Chemie (K)
4		water	water		18.0	504	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 59 - 522 (UniProt: Q21A49)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Val 9	-	expression tag
Gly 10	-	expression tag
Thr 11	-	expression tag
Asn 12	-	expression tag
Asp 13	-	expression tag
Ala 14	-	expression tag
Asn 15	-	expression tag
Ile 16	-	expression tag
Ala 17	-	expression tag
Asp 18	-	expression tag
Val 19	-	expression tag
Val 20	-	expression tag
Thr 21	-	expression tag
Lys 22	-	expression tag
Val 23	-	expression tag
Leu 24	-	expression tag
Gly 25	-	expression tag
Glu 26	-	expression tag
Tyr 27	-	expression tag
Gly 28	-	expression tag
Ala 29	-	expression tag
Pro 30	-	expression tag
Gly 31	-	expression tag
Ala 32	-	expression tag
Val 33	-	expression tag
Ser 34	-	expression tag
Val 35	-	expression tag
Ala 36	-	expression tag
Ala 37	-	expression tag
Leu 38	-	expression tag
Thr 39	-	expression tag
Ala 40	-	expression tag
Lys 41	-	expression tag
Ser 42	-	expression tag
Pro 43	-	expression tag
Asp 44	-	expression tag
Gly 45	-	expression tag
Lys 46	-	expression tag
Ser 47	-	expression tag
Asn 48	-	expression tag
Ser 49	-	expression tag
Ser 50	-	expression tag
Ala 51	-	expression tag
Asp 52	-	expression tag
Ala 53	-	expression tag
Asp 54	-	expression tag
Val 55	-	expression tag
Val 56	-	expression tag
Ala 57	-	expression tag
Arg 58	-	expression tag
Gly 222	Pro 164	engineered mutation
Arg 257	Ile 199	engineered mutation
Leu 261	Asn 203	engineered mutation
Leu 280	Ile 222	engineered mutation
Ile 326	Leu 268	engineered mutation
Thr 327	Pro 269	engineered mutation
Thr 329	Val 271	engineered mutation
His 481	Leu 423	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	552751.4
Non-Polymers*	Number of molecules	20
	Total formula weight	7825.0
All*	Total formula weight	560576.4

*Water molecules are not included.