6GTQ

Structure of the AtaT Y144F mutant toxin bound to the C-terminus of the antitoxin AtaR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acetyltransferase	polymer	178	19900.8	2	UniProt (A0A1V3CQ74)	Escherichia coli
2	C, D (C, D)	DUF1778 domain-containing protein	polymer	46	5254.1	2	UniProt (J7QA90) Pfam (PF08681)	Escherichia coli	Toxin-antitoxin system,antitoxin component,ribbon-helix-helix fold protein,Ybl13
3	E, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	F, K, L, M (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	G (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6	H, I, O, P, Q (A, B)	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
7	N (B)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
8	R (B)	IODIDE ION	non-polymer		126.9	1	Chemie (IOD)
9	S, T, U, V (A, B, C, D)	water	water		18.0	214	Chemie (HOH)

Sequence modifications

A, B: 1 - 174 (UniProt: A0A1V3CQ74)

PDB	External Database	Details
Met -3	-	initiating methionine
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Phe 143	Tyr 144	engineered mutation

C, D: 43 - 87 (UniProt: J7QA90)

PDB	External Database	Details
Met 42	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	50309.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1145.5
All*	Total formula weight	51455.3

*Water molecules are not included.