6GTQ
Structure of the AtaT Y144F mutant toxin bound to the C-terminus of the antitoxin AtaR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-22 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.580, 87.580, 125.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.240 - 2.490 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gtp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.065 | 1.321 |
Number of reflections | 25564 | |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.3 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1 M citrate, 20%(w/v) PEG 6000 |