6GRH

E. coli Microcin synthetase McbBCD complex with truncated pro-MccB17 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Bacteriocin microcin B17	polymer	54	5141.7	1	UniProt (P05834)	Escherichia coli str. K-12 substr. MG1655	MccB17
2	B, C (1, 2)	Microcin B17-processing protein McbB	polymer	295	34032.4	2	UniProt (P23184)	Escherichia coli str. K-12 substr. MG1655
3	D (C)	Microcin B17-processing protein McbC	polymer	272	30789.1	1	UniProt (P23185) Pfam (PF00881)	Escherichia coli str. K-12 substr. MG1655
4	E (D)	Microcin B17-processing protein McbD	polymer	396	44964.0	1	UniProt (P23186) Pfam (PF02624)	Escherichia coli str. K-12 substr. MG1655
5	F, K (1, 2)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	G (1)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	H, R (1, D)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	I, J, L, M, N... (1, 2, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
9	O (C)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
10	S (D)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
11	T, U, V, W, X (A, 1, 2, C, D)	water	water		18.0	512	Chemie (HOH)

Sequence modifications

A: 1 - 44 (UniProt: P05834)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Otz 39	Gly 39	modified residue
Otz 39	Ser 40	modified residue
Otz 39	Cys 41	modified residue

D: 1 - 396 (UniProt: P23186)

PDB	External Database	Details
Arg 171	Thr 171	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148959.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1280.7
All*	Total formula weight	150240.2

*Water molecules are not included.