6GRH
E. coli Microcin synthetase McbBCD complex with truncated pro-MccB17 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.740, 83.760, 87.120 |
| Unit cell angles | 90.00, 91.33, 90.00 |
Refinement procedure
| Resolution | 57.540 - 1.850 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6gos |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.540 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.086 | 1.435 |
| Number of reflections | 110695 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | NULL |
