6GPG

Structure of the RIG-I Singleton-Merten syndrome variant C268F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, C)	RNA (5'-R(*CP*GP*AP*CP*GP*CP*UP*AP*GP*CP*GP*UP*CP*G)-3')	polymer	14	4477.7	2		synthetic construct
2	C (A)	Probable ATP-dependent RNA helicase DDX58	polymer	714	81896.4	1	UniProt (O95786) Pfam (PF00270) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648)	Homo sapiens (Human)	DEAD box protein 58,RIG-I-like receptor 1,RLR-1,Retinoic acid-inducible gene 1 protein,RIG-1,Retinoic acid-inducible gene I protein,RIG-I
3	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 232 - 925 (UniProt: O95786)

PDB	External Database	Details
Met 212	-	initiating methionine
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
Ser 219	-	expression tag
Ser 220	-	expression tag
Gly 221	-	expression tag
Leu 222	-	expression tag
Glu 223	-	expression tag
Val 224	-	expression tag
Leu 225	-	expression tag
Phe 226	-	expression tag
Gln 227	-	expression tag
Gly 228	-	expression tag
Pro 229	-	expression tag
His 230	-	expression tag
Met 231	-	expression tag
Phe 268	Cys 268	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	90851.8
Non-Polymers*	Number of molecules	2
	Total formula weight	89.7
All*	Total formula weight	90941.5

*Water molecules are not included.