6GOV

Structure of THE RNA POLYMERASE LAMBDA-BASED ANTITERMINATION COMPLEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transcription termination/antitermination protein NusA	polymer	497	55030.8	1	UniProt (P0AFF8) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) UniProt (by SIFTS) (P0AFF6)	Escherichia coli O157:H7
2	B (B)	Transcription antitermination protein NusB	polymer	141	15838.2	1	UniProt (P0A782) Pfam (PF01029) UniProt (by SIFTS) (P0A780)	Escherichia coli O157:H7	Antitermination factor NusB
3	C (E)	30S ribosomal protein S10	polymer	106	12012.9	1	UniProt (P0A7R7) Pfam (PF00338) UniProt (by SIFTS) (P0A7R5)	Escherichia coli O157:H7
4	D (G)	Transcription termination/antitermination protein NusG	polymer	184	20831.8	1	UniProt (P0AFG1) Pfam (PF02357) Pfam (PF00467) UniProt (by SIFTS) (P0AFG0)	Escherichia coli O157:H7
5	E (N)	Antitermination protein N	polymer	110	12580.6	1	UniProt (P03045) Pfam (PF11438)	Escherichia phage lambda	Regulatory protein N,PN
6	F, G (U, V)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4)	Escherichia coli O157:H7	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
7	H (W)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800)	Escherichia coli O157:H7	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	I (X)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2)	Escherichia coli O157:H7	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
9	J (Y)	DNA-directed RNA polymerase subunit beta'	polymer	1417	156716.1	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7)	Escherichia coli O157:H7	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
10	K (K)	DNA (I)	polymer	65	20022.9	1		synthetic construct
11	L (L)	DNA (II)	polymer	65	19919.8	1		synthetic construct
12	M (R)	RNA (TRANSCRIPTION BUBBLE)	polymer	66	21238.7	1		synthetic construct
13	N (Y)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
14	O, P (Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 495 (UniProt: P0AFF8)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 358	Thr 358	conflict

B: 1 - 139 (UniProt: P0A782)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

E: 1 - 103 (UniProt: P0A7R7)

PDB	External Database	Details
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

G: 1 - 181 (UniProt: P0AFG1)

PDB	External Database	Details
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Glu 37	Asp 37	conflict

N: 1 - 107 (UniProt: P03045)

PDB	External Database	Details
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Y: 1 - 1407 (UniProt: P0A8T8)

PDB	External Database	Details
Val 1	Met 1	conflict
His 1408	-	expression tag
His 1409	-	expression tag
His 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag
His 1417	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	568379.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	568534.7

*Water molecules are not included.