6GH6

Cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme intermediate partially loaded complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (M)	RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor	polymer	497	54735.1	1	UniProt (A0A0J4U551) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223)	Klebsiella pneumoniae
6	G (N)	nifH promoter non-tmeplate DNA	polymer	63	19487.4	1		Klebsiella pneumoniae
7	H (T)	nifH promoter template DNA	polymer	63	19404.4	1		Klebsiella pneumoniae

Sequence modifications

M: 1 - 258 (UniProt: A0A0J4U551)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

M: 259 - 293 (PDB: 6GH6)
M: 294 - 397 (UniProt: A0A0J4U551)

PDB	External Database	Details
Ala 336	Arg 336	engineered mutation

M: 398 - 413 (PDB: 6GH6)
M: 414 - 477 (UniProt: A0A0J4U551)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	483181.5
All*	Total formula weight	483181.5

*Water molecules are not included.