6GDG

Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TrxA,Adenosine receptor A2a	polymer	479	52909.8	1	UniProt (Q14F07) UniProt (P29274) Pfam (PF00085) Pfam (PF00001) UniProt (by SIFTS) (P0AA25)	Escherichia coli
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (D)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
5	E (E)	nanobody Nb35	polymer	156	16926.1	1		Lama glama (Llama)
6	F (A)	N-ETHYL-5'-CARBOXAMIDO ADENOSINE	non-polymer		308.3	1	Chemie (NEC) PubChem (104795) PubChem (686179) PubChem (1254895) PubChem (5342881) PubChem (6541320) PubChem (6603839) PubChem (6604901) PubChem (6610317) PubChem (7068779) PubChem (7256363) PubChem (7256365) PubChem (11957539) PubChem (23913416) PubChem (24867503) PubChem (40967901) PubChem (40967902) PubChem (44308232) PubChem (44338050) PubChem (50987963) PubChem (2868392) PubChem (448222) PubChem (118705485) PubChem (124720172) PubChem (133688337) PubChem (138106599)

Sequence modifications

A: -106 - 1 (UniProt: Q14F07)

PDB	External Database	Details
Met -162	-	initiating methionine
Leu -161	-	expression tag
Leu -160	-	expression tag
Val -159	-	expression tag
Asn -158	-	expression tag
Gln -157	-	expression tag
Ser -156	-	expression tag
His -155	-	expression tag
Gln -154	-	expression tag
Gly -153	-	expression tag
Phe -152	-	expression tag
Asn -151	-	expression tag
Lys -150	-	expression tag
Glu -149	-	expression tag
His -148	-	expression tag
Thr -147	-	expression tag
Ser -146	-	expression tag
Lys -145	-	expression tag
Met -144	-	expression tag
Val -143	-	expression tag
Ser -142	-	expression tag
Ala -141	-	expression tag
Ile -140	-	expression tag
Val -139	-	expression tag
Leu -138	-	expression tag
Tyr -137	-	expression tag
Val -136	-	expression tag
Leu -135	-	expression tag
Leu -134	-	expression tag
Ala -133	-	expression tag
Ala -132	-	expression tag
Ala -131	-	expression tag
Ala -130	-	expression tag
His -129	-	expression tag
Ser -128	-	expression tag
Ala -127	-	expression tag
Phe -126	-	expression tag
Ala -125	-	expression tag
Ala -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
Glu -113	-	expression tag
Asn -112	-	expression tag
Leu -111	-	expression tag
Tyr -110	-	expression tag
Phe -109	-	expression tag
Gln -108	-	expression tag
Gly -107	-	expression tag
Ser -75	Cys 68	conflict
Ser -72	Cys 71	conflict
Ala -25	Lys 118	conflict
Glu 2	-	linker
Ala 3	-	linker
Ala 4	-	linker
Ala 5	-	linker
Lys 6	-	linker
Ala 7	-	linker

A: 8 - 316 (UniProt: P29274)

PDB	External Database	Details
Ala 154	Asn 154	conflict

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	conflict

D: 6 - 195 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

D: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
Ala 362	Ile 372	conflict
Ile 365	Val 375	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	143874.4
Non-Polymers*	Number of molecules	1
	Total formula weight	308.3
All*	Total formula weight	144182.6

*Water molecules are not included.