6GDG

Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	TrxA,Adenosine receptor A2a	polymer	479	52909.8	1	UniProt (Q14F07) UniProt (P29274) Pfam (PF00085) Pfam (PF00001) UniProt (by SIFTS) (P0AA25)	Escherichia coli
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (D)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
5	E (E)	nanobody Nb35	polymer	156	16926.1	1		Lama glama (Llama)
6	F (A)	N-ETHYL-5'-CARBOXAMIDO ADENOSINE	non-polymer		308.3	1	Chemie (NEC)

Sequence modifications

A: -106 - 1 (UniProt: Q14F07)

PDB	External Database	Details
Met -162	-	initiating methionine
Leu -161	-	expression tag
Leu -160	-	expression tag
Val -159	-	expression tag
Asn -158	-	expression tag
Gln -157	-	expression tag
Ser -156	-	expression tag
His -155	-	expression tag
Gln -154	-	expression tag
Gly -153	-	expression tag
Phe -152	-	expression tag
Asn -151	-	expression tag
Lys -150	-	expression tag
Glu -149	-	expression tag
His -148	-	expression tag
Thr -147	-	expression tag
Ser -146	-	expression tag
Lys -145	-	expression tag
Met -144	-	expression tag
Val -143	-	expression tag
Ser -142	-	expression tag
Ala -141	-	expression tag
Ile -140	-	expression tag
Val -139	-	expression tag
Leu -138	-	expression tag
Tyr -137	-	expression tag
Val -136	-	expression tag
Leu -135	-	expression tag
Leu -134	-	expression tag
Ala -133	-	expression tag
Ala -132	-	expression tag
Ala -131	-	expression tag
Ala -130	-	expression tag
His -129	-	expression tag
Ser -128	-	expression tag
Ala -127	-	expression tag
Phe -126	-	expression tag
Ala -125	-	expression tag
Ala -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
Glu -113	-	expression tag
Asn -112	-	expression tag
Leu -111	-	expression tag
Tyr -110	-	expression tag
Phe -109	-	expression tag
Gln -108	-	expression tag
Gly -107	-	expression tag
Ser -75	Cys 68	conflict
Ser -72	Cys 71	conflict
Ala -25	Lys 118	conflict
Glu 2	-	linker
Ala 3	-	linker
Ala 4	-	linker
Ala 5	-	linker
Lys 6	-	linker
Ala 7	-	linker

A: 8 - 316 (UniProt: P29274)

PDB	External Database	Details
Ala 154	Asn 154	conflict

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	conflict

D: 6 - 195 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

D: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
Ala 362	Ile 372	conflict
Ile 365	Val 375	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	143874.4
Non-Polymers*	Number of molecules	1
	Total formula weight	308.3
All*	Total formula weight	144182.6

*Water molecules are not included.