6GA0
Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with SNAP-Vista Green
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Methylated-DNA--protein-cysteine methyltransferase | polymer | 170 | 19242.4 | 1 | UniProt (Q97VW7) Pfam (PF01035) In PDB | Sulfolobus solfataricus P2 | 6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase |
2 | A | 5-[(4-methylphenyl)methylcarbamoyl]-2-(6-oxidanyl-3-oxidanylidene-4~{H}-xanthen-9-yl)benzoic acid | non-polymer | 479.5 | 1 | Chemie (ETW) | |||
3 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
4 | water | water | 18.0 | 95 | Chemie (HOH) |
Sequence modifications
A: 1 - 151 (UniProt: Q97VW7)
PDB | External Database | Details |
---|---|---|
Met -12 | - | initiating methionine |
Arg -11 | - | expression tag |
Gly -10 | - | expression tag |
Ser -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
Thr -2 | - | expression tag |
Asp -1 | - | expression tag |
Pro 0 | - | expression tag |
Ala 100 | Ser 100 | engineered mutation |
Ala 102 | Arg 102 | engineered mutation |
Lys 105 | Gly 105 | engineered mutation |
Thr 106 | Met 106 | engineered mutation |
Glu 110 | Lys 110 | engineered mutation |
Glu 132 | Ser 132 | engineered mutation |
Leu 152 | - | expression tag |
Gln 153 | - | expression tag |
Val 154 | - | expression tag |
Pro 155 | - | expression tag |
Ser 156 | - | expression tag |
Thr 157 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 19242.4 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 671.6 | |
All* | Total formula weight | 19914.0 |