6G1S

CryoEM structure of the MDA5-dsRNA filament with 87-degree helical twist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Interferon-induced helicase C domain-containing protein 1	polymer	693	79703.3	1	UniProt (Q8R5F7) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648) In PDB	Mus musculus (Mouse)	Helicase with 2 CARD domains,Helicard,Interferon induced with helicase C domain protein 1,Melanoma differentiation-associated protein 5,MDA-5,RIG-I-like receptor 2,RLR-2
2	X	RNA (5'-R(P*UP*CP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*G)-3')	polymer	15	4767.9	1		Pseudomonas phage phi6
3	Y	RNA (5'-R(P*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*GP*A)-3')	polymer	15	4807.9	1		Pseudomonas phage phi6
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 310 - 1020 (UniProt: Q8R5F7)

PDB	External Database	Details
-	Asp 597	deletion
-	Lys 598	deletion
-	Ser 599	deletion
-	Asp 600	deletion
-	Asp 601	deletion
-	Glu 602	deletion
-	Ala 603	deletion
-	Ser 604	deletion
-	Ser 605	deletion
-	Cys 606	deletion
-	Asn 607	deletion
-	Asp 608	deletion
-	Gln 609	deletion
-	Leu 610	deletion
-	Lys 611	deletion
-	Gly 612	deletion
-	Asp 613	deletion
-	Val 614	deletion

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	89279.1
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	89344.5

*Water molecules are not included.