6FZ6

Crystal Structure of a radical SAM methyltransferase from Sphaerobacter thermophilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Probable dual-specificity RNA methyltransferase RlmN	polymer	366	40696.8	2	UniProt (D1C4T7) Pfam (PF21016) Pfam (PF04055)	Sphaerobacter thermophilus (strain DSM 20745 / S 6022)	23S rRNA (adenine(2503)-C(2))-methyltransferase,23S rRNA m2A2503 methyltransferase,Ribosomal RNA large subunit methyltransferase N,tRNA (adenine(37)-C(2))-methyltransferase,tRNA m2A37 methyltransferase
2	C, G (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D, H (A, B)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
4	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	F, I (A, B)	BROMIDE ION	non-polymer		79.9	2	Chemie (BR)
6	J, K (A, B)	water	water		18.0	636	Chemie (HOH)

Sequence modifications

A, B: 1 - 347 (UniProt: D1C4T7)

PDB	External Database	Details
Met -18	-	initiating methionine
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81393.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1724.0
All*	Total formula weight	83117.6

*Water molecules are not included.