6FZ6
Crystal Structure of a radical SAM methyltransferase from Sphaerobacter thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 131.840, 53.140, 113.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.287 - 1.420 |
R-factor | 0.1671 |
Rwork | 0.166 |
R-free | 0.18710 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.109 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.010 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.132 | 2.691 |
Number of reflections | 150914 | |
<I/σ(I)> | 9.65 | 0.95 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.07 | |
CC(1/2) | 0.997 | 0.494 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide, 0.1 M HEPES/MOPS pH 7.5, 20 % Glycerol, 10% PEG4000 |