6FWQ

Structure of an E333Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-1,3-mannobiose and alpha-1,2-mannobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycosyl hydrolase family 71	polymer	385	43932.6	1	UniProt (D6D1V7) Pfam (PF16317)	Bacteroides xylanisolvens XB1A
2	B (B)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose	branched		342.3	1	In PDB BIRD (PRD_900112) GlyTouCan (G00891MP)		3alpha-alpha-mannobiose
3	C (C)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		342.3	1	In PDB BIRD (PRD_900111) GlyTouCan (G53402KW)		2alpha-alpha-mannobiose
4	D (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	E (A)	water	water		18.0	160	Chemie (HOH)

Sequence modifications

A: 17 - 380 (UniProt: D6D1V7)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag
Gln 333	Glu 333	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43932.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	44640.2

*Water molecules are not included.