6FWK
The crystal structure of Pol2CORE-M644G in complex with DNA and an incoming nucleotide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, B) | DNA polymerase epsilon catalytic subunit A | polymer | 1191 | 137101.5 | 2 | UniProt (P21951) Pfam (PF03104) Pfam (PF22634) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | DNA polymerase II subunit A |
| 2 | B, E (P, C) | DNA (5'-D(P*TP*AP*AP*CP*CP*GP*CP*GP*TP*TP*(DOC))-3') | polymer | 11 | 3293.2 | 2 | synthetic construct | ||
| 3 | C, F (T, D) | DNA (5'-D(P*TP*CP*TP*TP*GP*AP*AP*CP*GP*CP*GP*GP*TP*TP*A)-3') | polymer | 15 | 4600.0 | 2 | synthetic construct | ||
| 4 | G, K (A, B) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 2 | Chemie (DTP) PubChem (622) PubChem (15993) PubChem (44119995) PubChem (46936612) PubChem (57233913) PubChem (448808) PubChem (89346292) PubChem (91632917) PubChem (92341128) PubChem (92449119) PubChem (92449120) PubChem (129069956) PubChem (171699368) | |||
| 5 | H, I, L, M, N (A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) PubChem (271) | |||
| 6 | J, O (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) PubChem (29936) | |||
| 7 | P, Q (A, B) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 1186 (UniProt: P21951)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Gly 644 | Met 644 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 289989.2 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1294.4 | |
| All* | Total formula weight | 291283.7 |
