6FWK

The crystal structure of Pol2CORE-M644G in complex with DNA and an incoming nucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	DNA polymerase epsilon catalytic subunit A	polymer	1191	137101.5	2	UniProt (P21951) Pfam (PF03104) Pfam (PF22634)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	DNA polymerase II subunit A
2	B, E (P, C)	DNA (5'-D(P*TP*AP*AP*CP*CP*GP*CP*GP*TP*TP*(DOC))-3')	polymer	11	3293.2	2		synthetic construct
3	C, F (T, D)	DNA (5'-D(P*TP*CP*TP*TP*GP*AP*AP*CP*GP*CP*GP*GP*TP*TP*A)-3')	polymer	15	4600.0	2		synthetic construct
4	G, K (A, B)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	2	Chemie (DTP)
5	H, I, L, M, N (A, B)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
6	J, O (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
7	P, Q (A, B)	water	water		18.0	11	Chemie (HOH)

Sequence modifications

A, B: 1 - 1186 (UniProt: P21951)

PDB	External Database	Details
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Gly 644	Met 644	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	289989.2
Non-Polymers*	Number of molecules	9
	Total formula weight	1294.4
All*	Total formula weight	291283.7

*Water molecules are not included.