6FWK
The crystal structure of Pol2CORE-M644G in complex with DNA and an incoming nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 153.961, 70.342, 158.965 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 19.988 - 2.503 |
| R-factor | 0.2249 |
| Rwork | 0.223 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.988 | 19.988 | 2.570 |
| High resolution limit [Å] | 2.500 | 11.190 | 2.500 |
| Rmerge | 0.091 | 0.024 | 1.658 |
| Rmeas | 0.108 | 0.029 | 1.961 |
| Total number of observations | 371529 | ||
| Number of reflections | 108097 | 1319 | 7523 |
| <I/σ(I)> | 8.76 | 29.12 | 0.63 |
| Completeness [%] | 99.2 | 98.5 | 94.1 |
| Redundancy | 3.437 | 3.206 | 3.443 |
| CC(1/2) | 0.998 | 0.999 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 50mM MES, 150mM NaAc and 8% PEG20K |
