6FWG

Structure of an E333Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with tetramannoside yeast mannan fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycosyl hydrolase family 71	polymer	385	43932.6	1	UniProt (D6D1V7) Pfam (PF16317)	Bacteroides xylanisolvens XB1A
2	B (B)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-methyl alpha-D-mannopyranoside	branched		680.6	1	In PDB GlyTouCan (G71057YG)
3	C, D (A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
4	E (A)	water	water		18.0	336	Chemie (HOH)

Sequence modifications

A: 17 - 380 (UniProt: D6D1V7)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag
Gln 333	Glu 333	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43932.6
Branched	Number of molecules	1
	Total formula weight	680.6
Non-Polymers*	Number of molecules	2
	Total formula weight	118.1
All*	Total formula weight	44731.3

*Water molecules are not included.