6FRG
Crystal structure of G-1F mutant of Ssp DnaB Mini-Intein variant M86
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Replicative DNA helicase | polymer | 169 | 18976.9 | 1 | UniProt (Q55418) Pfam (PF14890) Pfam (PF03796) In PDB | Synechocystis sp. (strain PCC 6803 / Kazusa) | |
| 2 | A | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 2 | Chemie (1PE) | |||
| 3 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 4 | Chemie (PEG) | |||
| 5 | water | water | 18.0 | 221 | Chemie (HOH) |
Sequence modifications
A: -4 - 106 (UniProt: Q55418)
A: 112 - 159 (UniProt: Q55418)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | initiating methionine |
| Phe 0 | Gly 380 | engineered mutation |
| Ala 1 | Cys 381 | engineered mutation |
| Pro 18 | Ser 398 | engineered mutation |
| Gly 24 | Asp 404 | engineered mutation |
| Thr 58 | Ile 438 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 107 | - | linker |
| Pro 108 | - | linker |
| Glu 109 | - | linker |
| Ile 110 | - | linker |
| Glu 111 | - | linker |
| Pro 114 | Ser 769 | engineered mutation |
| Pro 122 | Ser 777 | engineered mutation |
| Leu 142 | Pro 797 | engineered mutation |
| Arg 143 | His 798 | engineered mutation |
| Ala 154 | Asn 809 | engineered mutation |
| Lys 160 | - | expression tag |
| Leu 161 | - | expression tag |
| Gly 162 | - | expression tag |
| Gly 163 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 18976.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1051.2 | |
| All* | Total formula weight | 20028.1 |
