6FRG
Crystal structure of G-1F mutant of Ssp DnaB Mini-Intein variant M86
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-03 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 71.472, 71.472, 87.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.935 - 1.535 |
R-factor | 0.1442 |
Rwork | 0.143 |
R-free | 0.17010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mi8 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.669 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.940 | 43.940 | 1.560 |
High resolution limit [Å] | 1.535 | 8.410 | 1.535 |
Rmerge | 0.066 | 0.024 | 1.648 |
Rmeas | 0.068 | 0.025 | 1.701 |
Rpim | 0.017 | 0.007 | 0.418 |
Number of reflections | 34745 | 274 | 1683 |
<I/σ(I)> | 24.5 | ||
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 15.8 | 12.8 | 16.3 |
CC(1/2) | 1.000 | 1.000 | 0.646 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1 M sodium citrate pH 5.3, 48 % PEG200 |