6FIZ

Crystal Structure of CNG mimicking NaK-EAPP mutant (T67A) cocrystallized with K+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Potassium channel protein,Cyclic nucleotide-gated olfactory channel,Potassium channel protein	polymer	94	10294.1	16	UniProt (Q81HW2) UniProt (Q16280) Pfam (PF07885)	Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)	Cyclic nucleotide-gated cation channel 2,Cyclic nucleotide-gated channel alpha-2,CNG2
2	AA, BA, BB, BC, CA... (D, J, N, E, O...)	GLYCINE	non-polymer		75.1	60	Chemie (GLY)
3	AB, IA, JA, KA, S... (I, E, A, M)	POTASSIUM ION	non-polymer		39.1	12	Chemie (K)
4	AC, DA, JC, KB, LB... (M, D, N, J, F...)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	7	Chemie (MPD)
5	AD, BD, CD, DD, ED... (J, K, L, M, N...)	water	water		18.0	95	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P: 20 - 64 (UniProt: Q81HW2)

PDB	External Database	Details
Ala 19	-	expression tag

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P: 65 - 70 (UniProt: Q16280)

PDB	External Database	Details
Ala 67	Thr 341	engineered mutation

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P: 71 - 109 (UniProt: Q81HW2)

PDB	External Database	Details
Val 110	-	expression tag
Val 111	-	expression tag
Pro 112	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	164705.8
Non-Polymers*	Number of molecules	79
	Total formula weight	5800.4
All*	Total formula weight	170506.2

*Water molecules are not included.