6FHM

Crystal structure of the F47E mutant of the lipoprotein localization factor, LolA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Outer-membrane lipoprotein carrier protein	polymer	206	22917.1	2	UniProt (P61316) Pfam (PF03548)	Escherichia coli (strain K12)	P20
2	C, D, E (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	F, G (A, B)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A, B: 1 - 182 (UniProt: P61316)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Leu -10	-	expression tag
Val -9	-	expression tag
Pro -8	-	expression tag
Arg -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Glu 47	Phe 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45834.3
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	46110.6

*Water molecules are not included.