6FHM

Crystal structure of the F47E mutant of the lipoprotein localization factor, LolA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 2 21 21
Unit cell lengths	61.030, 77.600, 103.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.110 - 2.390
R-factor	0.21746
Rwork	0.215
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iwl
RMSD bond length	0.014
RMSD bond angle	1.666
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.110	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.196
Rmeas	0.215
Rpim	0.086	0.510
Number of reflections	20165
<I/σ(I)>	6.7
Completeness [%]	100.0
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	13% PEG 8000, 20% glycerol