6FHM
Crystal structure of the F47E mutant of the lipoprotein localization factor, LolA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.030, 77.600, 103.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.110 - 2.390 |
| R-factor | 0.21746 |
| Rwork | 0.215 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.666 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.110 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.196 | |
| Rmeas | 0.215 | |
| Rpim | 0.086 | 0.510 |
| Number of reflections | 20165 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 13% PEG 8000, 20% glycerol |
