6FE8

Cryo-EM structure of the core Centromere Binding Factor 3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Centromere DNA-binding protein complex CBF3 subunit B	polymer	584	68454.1	2	UniProt (P40969) Pfam (PF16846) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Centromere protein 3
2	C	Suppressor of kinetochore protein 1	polymer	197	22558.5	1	UniProt (P52286) Pfam (PF03931) Pfam (PF01466) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Centromere DNA-binding protein complex CBF3 subunit D,E3 ubiquitin ligase complex SCF subunit SKP1
3	D	Centromere DNA-binding protein complex CBF3 subunit C	polymer	519	60900.0	1	UniProt (P35203) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Chromosome transmission fidelity protein 13,Kinetochore protein CTF13

Sequence modifications

A, B: 47 - 608 (UniProt: P40969)

PDB	External Database	Details
Met 25	-	initiating methionine
Gly 26	-	expression tag
Gly 27	-	expression tag
Ser 28	-	expression tag
Ser 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
Ser 36	-	expression tag
Ser 37	-	expression tag
Gly 38	-	expression tag
Leu 39	-	expression tag
Val 40	-	expression tag
Pro 41	-	expression tag
Arg 42	-	expression tag
Gly 43	-	expression tag
Ser 44	-	expression tag
His 45	-	expression tag
Met 46	-	expression tag

C: 2 - 194 (UniProt: P52286)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Gly 195	-	expression tag
Ser 196	-	expression tag

D: 2 - 478 (UniProt: P35203)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Gly 479	-	expression tag
Ser 480	-	expression tag
Arg 481	-	expression tag
Ser 482	-	expression tag
Gly 483	-	expression tag
Ser 484	-	expression tag
Glu 485	-	expression tag
Asn 486	-	expression tag
Leu 487	-	expression tag
Tyr 488	-	expression tag
Phe 489	-	expression tag
Gln 490	-	expression tag
Gly 491	-	expression tag
Ser 492	-	expression tag
Lys 493	-	expression tag
Arg 494	-	expression tag
Arg 495	-	expression tag
Trp 496	-	expression tag
Lys 497	-	expression tag
Lys 498	-	expression tag
Asn 499	-	expression tag
Phe 500	-	expression tag
Ile 501	-	expression tag
Ala 502	-	expression tag
Val 503	-	expression tag
Ser 504	-	expression tag
Ala 505	-	expression tag
Ala 506	-	expression tag
Asn 507	-	expression tag
Arg 508	-	expression tag
Phe 509	-	expression tag
Lys 510	-	expression tag
Lys 511	-	expression tag
Ile 512	-	expression tag
Ser 513	-	expression tag
Ser 514	-	expression tag
Ser 515	-	expression tag
Gly 516	-	expression tag
Ala 517	-	expression tag
Leu 518	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	220366.7
All*	Total formula weight	220366.7

*Water molecules are not included.