6FC3

Crystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Eukaryotic translation initiation factor 4E	polymer	183	20806.4	1	UniProt (P07260) Pfam (PF01652)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	eIF4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein
2	B (B)	Cap-associated protein CAF20	polymer	52	6202.1	1	UniProt (P12962) Pfam (PF17052)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	20 kDa cap-associated protein,CCR4-associated factor 2,p20
3	C, E (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	F, G (A, B)	water	water		18.0	210	Chemie (HOH)

Sequence modifications

A: 35 - 213 (UniProt: P07260)

PDB	External Database	Details
Gly 31	-	expression tag
Pro 32	-	expression tag
His 33	-	expression tag
Met 34	-	expression tag
Ala 42	Lys 42	engineered mutation
Ala 168	Lys 168	engineered mutation
Ala 187	Lys 187	engineered mutation

B: 1 - 49 (UniProt: P12962)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27008.5
Non-Polymers*	Number of molecules	3
	Total formula weight	222.9
All*	Total formula weight	27231.4

*Water molecules are not included.