6FC3

Crystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	C 1 2 1
Unit cell lengths	102.016, 62.755, 44.732
Unit cell angles	90.00, 106.99, 90.00

Refinement procedure

Resolution	42.780 - 1.750
R-factor	0.1832
Rwork	0.181
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fbz chain A
RMSD bond length	0.020
RMSD bond angle	1.031
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	27294	1510
<I/σ(I)>	8.8	1.2
Completeness [%]	99.9	99.9
Redundancy	4.2	4.1
CC(1/2)		0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Na-acetate (pH 5.0) 13% (w/v) PEG 6000 10 mM ZnCl2