6F9G

Ligand binding domain of P. putida KT2440 polyamine chemorecpetors McpU in complex putrescine.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Methyl-accepting chemotaxis protein McpU	polymer	321	34732.8	5	UniProt (Q88NI1) Pfam (PF22673) In PDB	Pseudomonas putida KT2440
2	A, B, C, D, E	1,4-DIAMINOBUTANE	non-polymer		88.2	5	Chemie (PUT)
3	A, D, E	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	C, D	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5		water	water		18.0	296	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 33 - 334 (UniProt: Q88NI1)

PDB	External Database	Details
Met 14	-	initiating methionine
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Val 28	-	expression tag
Pro 29	-	expression tag
Arg 30	-	expression tag
Gly 31	-	expression tag
Ser 32	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	173664.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1019.3
All*	Total formula weight	174683.5

*Water molecules are not included.