6F89

Structure of H234A/Y235A P.abyssi Sua5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Threonylcarbamoyl-AMP synthase	polymer	346	38169.2	2	UniProt (Q9UYB2) Pfam (PF01300) Pfam (PF03481) In PDB	Pyrococcus abyssi (strain GE5 / Orsay)	TC-AMP synthase,L-threonylcarbamoyladenylate synthase,t(6)A37 threonylcarbamoyladenosine biosynthesis protein Sua5,tRNA threonylcarbamoyladenosine biosynthesis protein Sua5
2	A, B	BICARBONATE ION	non-polymer		61.0	2	Chemie (BCT)
3	A, B	THREONINE	non-polymer		119.1	2	Chemie (THR)
4		water	water		18.0	135	Chemie (HOH)

Sequence modifications

A, B: 1 - 340 (UniProt: Q9UYB2)

PDB	External Database	Details
Ala 234	His 234	engineered mutation
Ala 235	Tyr 235	engineered mutation
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76338.4
Non-Polymers*	Number of molecules	4
	Total formula weight	360.3
All*	Total formula weight	76698.7

*Water molecules are not included.