6F89
Structure of H234A/Y235A P.abyssi Sua5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.440, 125.270, 47.940 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 47.920 - 2.810 |
| R-factor | 0.1644 |
| Rwork | 0.162 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eqa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.980 |
| High resolution limit [Å] | 2.810 | 2.811 |
| Rmeas | 0.074 | |
| Number of reflections | 24980 | |
| <I/σ(I)> | 9.22 | |
| Completeness [%] | 97.8 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate |
