6EZT

Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-N-acetylglucosaminidase Nag2	polymer	649	74254.6	2	UniProt (D9ISE0) Pfam (PF02838) Pfam (PF00728) In PDB	Vibrio harveyi (Beneckea harveyi)
2	A, B	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
3	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4		water	water		18.0	424	Chemie (HOH)

Sequence modifications

A, B: 4 - 642 (UniProt: D9ISE0)

PDB	External Database	Details
Ala 437	Asp 437	engineered mutation
Arg 643	-	expression tag
Ser 644	-	expression tag
Arg 645	-	expression tag
Ser 646	-	expression tag
His 647	-	expression tag
His 648	-	expression tag
His 649	-	expression tag
His 650	-	expression tag
His 651	-	expression tag
His 652	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	148509.2
Non-Polymers*	Number of molecules	4
	Total formula weight	600.7
All*	Total formula weight	149109.9

*Water molecules are not included.