6ET2

Crystal structure of PqsBC (C129A) mutant from Pseudomonas aeruginosa (crystal form 3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	PqsC	polymer	365	40321.8	8	UniProt (Q9I4X1) Pfam (PF08545) Pfam (PF08541)	Pseudomonas aeruginosa PAO1
2	B, D, F, H, J... (B, D, F, H, J...)	PqsB	polymer	283	30531.8	8	UniProt (Q9I4X2)	Pseudomonas aeruginosa PAO1
3	Q, R, S, T, U... (A, C, E, G, I...)	3[N-MORPHOLINO]PROPANE SULFONIC ACID	non-polymer		209.3	8	Chemie (MPO)
4	AA, BA, CA, DA, EA... (C, D, E, F, G...)	water	water		18.0	825	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O: 1 - 348 (UniProt: Q9I4X1)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ala -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 129	Cys 129	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	566828.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1674.1
All*	Total formula weight	568502.4

*Water molecules are not included.